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Engineering of vesicle trafficking improves heterologous protein secretion in Saccharomyces cerevisiae

Paper ID Volume ID Publish Year Pages File Format Full-Text
31581 44821 2012 8 PDF Available
Title
Engineering of vesicle trafficking improves heterologous protein secretion in Saccharomyces cerevisiae
Abstract

The yeast Saccharomyces cerevisiae is a widely used platform for the production of heterologous proteins of medical or industrial interest. However, heterologous protein productivity is often restricted due to the limitations of the host strain. In the protein secretory pathway, the protein trafficking between different organelles is catalyzed by the soluble NSF (N-ethylmaleimide-sensitive factor) receptor (SNARE) complex and regulated by the Sec1/Munc18 (SM) proteins. In this study, we report that over-expression of the SM protein encoding genes SEC1 and SLY1, improves the protein secretion in S. cerevisiae. Engineering Sec1p, the SM protein that is involved in vesicle trafficking from Golgi to cell membrane, improves the secretion of heterologous proteins human insulin precursor and α-amylase, and also the secretion of an endogenous protein invertase. Enhancing Sly1p, the SM protein regulating the vesicle fusion from endoplasmic reticulum (ER) to Golgi, increases α-amylase production only. Our study demonstrates that strengthening the protein trafficking in ER-to-Golgi and Golgi-to-plasma membrane process is a novel secretory engineering strategy for improving heterologous protein production in S. cerevisiae.

► Engineering vesicle trafficking to study the effects of heterologous protein production. ► Over expression Sec1p improves insulin precursor, α-amylase and invertase secretion. ► Over expression Sly1p increases α-amylase production. ► Physiological properties of protein strains are characterized.

Keywords
Vesicle trafficking; Sec1p; Sly1p; Heterologous protein secretion; Saccharomyces cerevisiae
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Engineering of vesicle trafficking improves heterologous protein secretion in Saccharomyces cerevisiae
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Publisher
Database: Elsevier - ScienceDirect
Journal: Metabolic Engineering - Volume 14, Issue 2, March 2012, Pages 120–127
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us