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Adaptively evolved yeast mutants on galactose show trade-offs in carbon utilization on glucose

Paper ID Volume ID Publish Year Pages File Format Full-Text
31596 44823 2013 9 PDF Available
Title
Adaptively evolved yeast mutants on galactose show trade-offs in carbon utilization on glucose
Abstract

Adaptive evolution offers many opportunities in metabolic engineering; however, several constraints still exist as evolutionary trade-offs may impose collateral cost to obtain new traits. The application of adaptive evolution for strains development could be further improved by elucidating the molecular mechanisms. In this study, adaptively evolved yeast mutants with improved galactose utilization ability showed impaired glucose utilization. The molecular genetic basis of this trade-off was investigated using a systems biology approach. Transcriptional and metabolic changes resulting from the improvement of galactose utilization were found maintained during growth on glucose. Moreover, glucose repression related genes showed conserved expression patterns during growth on both sugars. Mutations in the RAS2 gene that were identified as beneficial for galactose utilization in evolved mutants exhibited significant correlation with attenuation of glucose utilization. These results indicate that antagonistic pleiotropy is the dominant mechanism in the observed trade-off, and it is likely realized by changes in glucose signaling.

► Genetic context in evolutionary engineering for strain development. ► Molecular and genetic bases of evolutionary trade-off in carbon utilization. ► Antagonistic pleiotropy by attenuation of glucose regulation.

Keywords
Metabolic engineering; Evolutionary trade-off; Systems biology; Galactose metabolism; Glucose regulation; Ras/PKA signaling pathway
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Adaptively evolved yeast mutants on galactose show trade-offs in carbon utilization on glucose
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Publisher
Database: Elsevier - ScienceDirect
Journal: Metabolic Engineering - Volume 16, March 2013, Pages 78–86
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us