fulltext.study @t Gmail

Metabolic engineering of the flavone-C-glycoside pathway using polyprotein technology

Paper ID Volume ID Publish Year Pages File Format Full-Text
31602 44823 2013 10 PDF Available
Title
Metabolic engineering of the flavone-C-glycoside pathway using polyprotein technology
Abstract

C-Glycosylated flavonoids are biologically active plant natural products linked to dietary health benefits. We have used polyprotein expression technology to reconstruct part of the respective biosynthetic pathway in tobacco and yeast, such that dihydrochalcone and flavanone precursors are directly converted to C-glycosides. The polyprotein system developed facilitated the simple and efficient co-expression of pathway enzymes requiring different sub-cellular localization in both plants and yeast. The pathway to flavone-C-glucosides comprised a flavanone 2-hydroxylase (F2H), co-expressed with a C-glucosyltransferase (CGT). While pathway engineering in tobacco resulted in only minor C-glycoside formation, when fed with the flavanone naringenin, yeast transformed with the F2H-CGT polyprotein construct produced high concentrations of 2-hydroxynaringenin-C-glucoside in the medium. These fermentation products could then be readily chemically converted to the respective flavone-C-glucosides. The efficiency of the biosynthesis was optimal when both the F2H and CGT were obtained from the same species (rice). These results confirm the coupled roles of the F2H and CGT in producing C-glucosides in vivo, with the use of the polyprotein expression system in yeast offering a useful system to optimize the synthesis of these natural products in quantities suitable for dietary studies.

► Production platform for biosynthesis of C-glycosides developed in yeast. ► Polyprotein technology used to co-express cytochrome P450 and C-glycosyltransferase. ► Significant quantities of biologically active C-glycosides produced in medium.

Keywords
CGT, C-glucosyltransferase; F2H, flavanone 2-hydroxylase; CPR, cytochrome P450 reductase; FNSII, flavone synthase II; IFS, isoflavone synthaseC-glucosyltransferase; Dihydrochalcones; Cytochrome P450; Rice (Oryza sativa); Yeast (Saccharomyces cerevisiae)
First Page Preview
Metabolic engineering of the flavone-C-glycoside pathway using polyprotein technology
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us
Publisher
Database: Elsevier - ScienceDirect
Journal: Metabolic Engineering - Volume 16, March 2013, Pages 11–20
Authors
, ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us