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Metabolic engineering in strawberry fruit uncovers a dormant biosynthetic pathway

Paper ID Volume ID Publish Year Pages File Format Full-Text
31612 44824 2011 5 PDF Available
Title
Metabolic engineering in strawberry fruit uncovers a dormant biosynthetic pathway
Abstract

Wild strawberry (Fragaria vesca) fruit contains several important phenylpropene aroma compounds such as eugenol, but cultivated varieties are mostly devoid of them. We have redirected the carbon flux in cultivated strawberry (Fragaria×ananassa) fruit from anthocyanin pigment biosynthesis to the production of acetates of hydroxycinnamyl alcohols, which serve as the precursors of the phenylpropenes, by downregulating the strawberry chalcone synthase (CHS) via RNAi-mediated gene silencing and, alternatively, by an antisense CHS construct. Simultaneous heterologous overexpression of a eugenol (EGS) and isoeugenol synthase (IGS) gene in the same cultivated strawberry fruits boosted the formation of eugenol, isoeugenol, and the related phenylpropenes chavicol and anol to concentrations orders of magnitude greater than their odor thresholds. The results show that Fragaria×ananassa still bears a phenylpropene biosynthetic pathway but the carbon flux is primarily directed to the formation of pigments. Thus, partial restoration of wild strawberry flavor in cultivated varieties is feasible by diverting the flavonoid pathway to phenylpropene synthesis through metabolic engineering.

► Wild strawberry (Fragaria vesca) fruit contains aroma compounds such as eugenol. ► Cultivated varieties (Fragaria×ananassa) are mostly devoid of them. ► The carbon flux in cultivated strawberry fruit has been redirected to form eugenol. ► The chalcone synthase was silenced and a eugenol synthase was overexpressed. ► Fragaria×ananassa still bears a dormant phenylpropene biosynthetic pathway.

Keywords
Eugenol synthase; Isoeugenol synthase; Chalcone synthase; Phenylpropanoids; Flavonoids; Fragaria×ananassa
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Metabolic engineering in strawberry fruit uncovers a dormant biosynthetic pathway
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Publisher
Database: Elsevier - ScienceDirect
Journal: Metabolic Engineering - Volume 13, Issue 5, September 2011, Pages 527–531
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us