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Styrene biosynthesis from glucose by engineered E. coli

Paper ID Volume ID Publish Year Pages File Format Full-Text
31614 44824 2011 11 PDF Available
Title
Styrene biosynthesis from glucose by engineered E. coli
Abstract

Styrene is a large volume, commodity petrochemical with diverse commercial applications, including as a monomer building-block for the synthesis of many useful polymers. Here we demonstrate how, through the de novo design and development of a novel metabolic pathway, styrene can alternatively be synthesized from renewable substrates such as glucose. The conversion of endogenously synthesized l-phenylalanine to styrene was achieved by the co-expression of phenylalanine ammonia lyase and trans-cinnamate decarboxylase. Candidate isoenzymes for each step were screened from bacterial, yeast, and plant genetic sources. Finally, over-expression of PAL2 from Arabidopsis thaliana and FDC1 from Saccharomyces cerevisiae (originally classified as ferulate decarboxylase) in an l-phenylalanine over-producing Escherichia coli host led to the accumulation of up to 260 mg/L in shake flask cultures. Achievable titers already approach the styrene toxicity threshold (determined as ∼300 mg/L). To the best of our knowledge, this is the first report of microbial styrene production from sustainable feedstocks.

► E. coli has been engineered to produce styrene from renewable resources. ► The styrene biosynthesis pathway was constructed using genes from plants, yeast, and bacteria. ► Pathway enzymes were systematically selected based on recombinant activity. ► Preliminary titers already approach the styrene toxicity limit of 300 mg/L.

Keywords
Styrene; Phenylalanine ammonia lyase; E. coli; Cinnamic acid; l-Phenylalanine; Aromatic
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Styrene biosynthesis from glucose by engineered E. coli
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Publisher
Database: Elsevier - ScienceDirect
Journal: Metabolic Engineering - Volume 13, Issue 5, September 2011, Pages 544–554
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
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Full-text PDF Download
Online Support
Any Questions? feel free to contact us