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Engineering the robustness of Clostridium acetobutylicum by introducing glutathione biosynthetic capability

Paper ID Volume ID Publish Year Pages File Format Full-Text
31639 44826 2011 9 PDF Available
Title
Engineering the robustness of Clostridium acetobutylicum by introducing glutathione biosynthetic capability
Abstract

To improve the aero- and solvent tolerance of the solvent-producing Clostridium acetobutylicum, glutathione biosynthetic capability was introduced into C. acetobutylicum DSM1731 by cloning and over-expressing the gshAB genes from Escherichia coli. Strain DSM1731(pITAB) produces glutathione, and shows a significantly improved survival upon aeration and butanol challenge, as compared with the control. In addition, strain DSM1731(pITAB) exhibited an improved butanol tolerance and an increased butanol production capability, as compared with the recombinant strains with only gshA or gshB gene. These results illustrated that introducing glutathione biosynthetic pathway, which is redundant for the metabolism of C. acetobutylicum, can increase the robustness of the host to achieve a better solvent production.

Keywords
Clostridium acetobutylicum; Robustness; Glutathione; Aero-tolerance; Butanol tolerance; Butanol productionγ-GC, γ-glutamyl-cysteine; γ-GCS, γ-glutamylcysteine synthetase; CFE, cell free extracts; CFU, colony forming unit; CGM, clostridium glucose medium;
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Publisher
Database: Elsevier - ScienceDirect
Journal: Metabolic Engineering - Volume 13, Issue 4, July 2011, Pages 426–434
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
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Price was $35.95
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Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
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