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Application of a two-dimensional disposable rocking bioreactor to bacterial cultivation for recombinant protein production

Paper ID Volume ID Publish Year Pages File Format Full-Text
3164 153 2014 8 PDF Available
Title
Application of a two-dimensional disposable rocking bioreactor to bacterial cultivation for recombinant protein production
Abstract

•Comparison of various types of bioreactor systems.•Study of mixing and mass transfer issues associated with bioreactors.•Application of a 2-dimensional wave bioreactor system to bacterial cultivation for recombinant protein production.

Disposable rocking bioreactors (RBs) are widely employed for cultivation of recombinant mammalian and insect cell lines, although the perception of inadequate mass transfer has prevented their application to bioprocesses based on microbial platforms. In this study, one-dimensional (1D) and two-dimensional (2D) RBs were assessed and compared with the conventional stirred tank reactor (STR) for recombinant therapeutic protein production in Escherichia coli. The comparison involved: (1) physical characterization of oxygen mass transfer efficiency and mixing intensity, (2) growth characteristics in batch cultivation, and (3) culture performance for the production of recombinant protein. Our results show that oxygen mass transfer was comparable between the 1D RB and STR at low working volume (WV), declining linearly with increasing WV, and was highest in the 2D RB for all tested WVs with the maximum mass transfer coefficient (kLa) at 3 L WV. Well mixing behavior was observed in all three systems for water and aqueous carboxymethylcellulose (CMC) solutions. Batch growth characteristics were similar in all bioreactor systems, although metabolite accumulation was significant in the 1D RB. Culture performance for the production of recombinant GST-hCD83ext (glutathione S-transferase-hCD83ext fusion protein) was similar in terms of soluble protein yield and inclusion body formation for all bioreactor systems.

Keywords
Disposable bioreactor; Escherichia coli; Human CD83 (hCD83); Recombinant protein production; Rocking bioreactor; Therapeutic protein
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Application of a two-dimensional disposable rocking bioreactor to bacterial cultivation for recombinant protein production
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 88, 15 July 2014, Pages 154–161
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us