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Carotenoid-based phenotypic screen of the yeast deletion collection reveals new genes with roles in isoprenoid production

Paper ID Volume ID Publish Year Pages File Format Full-Text
31661 44827 2013 10 PDF Available
Title
Carotenoid-based phenotypic screen of the yeast deletion collection reveals new genes with roles in isoprenoid production
Abstract

Beside their essential cellular functions, isoprenoids have value as pharmaceuticals, nutriceuticals, pesticides, and fuel alternatives. Engineering microorganisms for production of isoprenoids is relatively easy, sustainable, and cost effective in comparison to chemical synthesis or extraction from natural producers. We introduced genes encoding carotenoid biosynthetic enzymes into the haploid yeast deletion collection to identify gene deletions that improved isoprenoid production. Deletions that showed significant improvement in carotenoid production were further screened for production of bisabolene, an isoprenoid alternative to petroleum-derived diesel. Combining those deletions with other mevalonate pathway modifications increased production of bisabolene from 40 mg/L to 800 mg/L in shake-flask cultures. In a fermentation process, this engineered strain produced 5.2 g/L of bisabolene.

► Yeast deletion collection was screened for improvements in carotenoid production. ► 24 of the highest carotenoid producing strains were tested for bisabolene production. ► Three gene deletions showed enhanced bisabolene production. ► These gene deletions and other modifications increased bisabolene production 20-fold. ► The best producing strain yielded 5.2 g/L of bisabolene in bioreactor experiment.

Keywords
Isoprenoids; S. cerevisiae; Carotenoids; Biofuel; Bisabolene
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Carotenoid-based phenotypic screen of the yeast deletion collection reveals new genes with roles in isoprenoid production
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Publisher
Database: Elsevier - ScienceDirect
Journal: Metabolic Engineering - Volume 15, January 2013, Pages 174–183
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us