fulltext.study @t Gmail

Metabolic and bioprocess engineering for production of selenized yeast with increased content of seleno-methylselenocysteine

Paper ID Volume ID Publish Year Pages File Format Full-Text
31671 44828 2011 12 PDF Available
Title
Metabolic and bioprocess engineering for production of selenized yeast with increased content of seleno-methylselenocysteine
Abstract

Specific Se-metabolites have been recognized to be the main elements responsible for beneficial effects of Se-enriched diet, and Se-methylselenocysteine (SeMCys) is thought to be among the most effective ones. Here we show that an engineered Saccharomyces cerevisiae strain, expressing a codon optimized heterologous selenocysteine methyltransferase and endowed with high intracellular levels of S-adenosyl-methionine, was able to accumulate SeMCys at levels higher than commercial selenized yeasts. A fine tuned carbon- and sulfate-limited fed-batch bioprocess was crucial to achieve good yields of biomass and SeMCys. Through the coupling of metabolic and bioprocess engineering we achieved a ∼24-fold increase in SeMCys, compared to certified reference material of selenized yeast. In addition, we investigated the interplay between sulfur and selenium metabolism and the possibility that redox imbalance occurred along with intracellular accumulation of Se. Collectively, our data show how the combination of metabolic and bioprocess engineering can be used for the production of selenized yeast enriched with beneficial Se-metabolites.

Keywords
Yeast; Seleno-methylselenocysteine; Selenium and sulfur metabolism; Mass spectrometry; Fed-batch; Metabolic engineeringSeCys, selenocysteine; SeMCys, seleno-methylselenocysteine; SeMet, selenomethionine; γ-glu-SeMCys, gamma-glutamyl-seleno-methylselenocys
First Page Preview
Metabolic and bioprocess engineering for production of selenized yeast with increased content of seleno-methylselenocysteine
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us
Publisher
Database: Elsevier - ScienceDirect
Journal: Metabolic Engineering - Volume 13, Issue 3, May 2011, Pages 282–293
Authors
, , , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us