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Selective reduction of xylose to xylitol from a mixture of hemicellulosic sugars

Paper ID Volume ID Publish Year Pages File Format Full-Text
31684 44829 2010 7 PDF Available
Title
Selective reduction of xylose to xylitol from a mixture of hemicellulosic sugars
Abstract

The biocatalytic reduction of d-xylose to xylitol requires separation of the substrate from l-arabinose, another major component of hemicellulosic hydrolysate. This step is necessitated by the innate promiscuity of xylose reductases, which can efficiently reduce l-arabinose to l-arabinitol, an unwanted byproduct. Unfortunately, due to the epimeric nature of d-xylose and l-arabinose, separation can be difficult, leading to high production costs. To overcome this issue, we engineered an E. coli strain to efficiently produce xylitol from d-xylose with minimal production of l-arabinitol byproduct. By combining this strain with a previously engineered xylose reductase mutant, we were able to eliminate l-arabinitol formation and produce xylitol to near 100% purity from an equiweight mixture of d-xylose, l-arabinose, and d-glucose.

Keywords
Xylitol; Arabinitol; Xylose reductase; Selectivity; Biocatalysis; Hemicellulose
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Selective reduction of xylose to xylitol from a mixture of hemicellulosic sugars
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Publisher
Database: Elsevier - ScienceDirect
Journal: Metabolic Engineering - Volume 12, Issue 5, September 2010, Pages 462–468
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us