fulltext.study @t Gmail

Protocorm culture of Dendrobium candidum in balloon type bubble bioreactors

Paper ID Volume ID Publish Year Pages File Format Full-Text
3169 153 2014 4 PDF Available
Title
Protocorm culture of Dendrobium candidum in balloon type bubble bioreactors
Abstract

•Dendrobium candidum protocorm suspension cultures in balloon type bubble bioreactors have been established.•Enhanced accumulation of biomass and bioactive compounds in bioreactor cultures was achieved.•The results of the current study are useful for the production of D. candidum bioactive compounds.

Protocorm cultures of Dendrobium candidum were established in balloon type bubble bioreactors using Murashige and Skoog (MS) medium with 0.5 mg l−1 α-naphthaleneacetic acid (NAA), 2.5% (w/v) sucrose, 5:25 mM NH4:NO3 and 1% (v/v) banana homogenate for the production of biomass and bioactive compounds. In 3 l bioreactor containing 2 l medium, a maximum protocorm biomass (21.0 g l−1 dry biomass) and also optimum quantities of total polysaccharides (389.3 mg g−1 DW), coumarins (18.0 mg g−1 DW), polyphenolics (11.9 mg g−1 DW), and flavonoids (4.5 mg g−1 DW) were achieved after 7 weeks of culture. Based on these studies, 5 and 10 l bioreactor cultures were established to harvest 80 g and 160 g dry biomass. In 10 l bioreactors, the protocorms grown were accumulated with optimal levels of polysaccharides (424.1 mg g−1 DW), coumarins (15.8 mg g−1 DW), polyphenols (9.03 mg g−1 DW) and flavonoids (4.7 mg g−1 DW). The bioreactor technology developed here will be useful for the production of important bioactive compounds from D. candidum.

Graphical abstractFigure optionsDownload full-size imageDownload as PowerPoint slide

Keywords
Bioreactors; Bioactive compounds; Biosynthesis; Plant cell cultures; Secondary metabolites
First Page Preview
Protocorm culture of Dendrobium candidum in balloon type bubble bioreactors
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us
Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 88, 15 July 2014, Pages 26–29
Authors
, , , , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us