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Metabolic engineering of Saccharomyces cerevisiae for the biotechnological production of succinic acid

Paper ID Volume ID Publish Year Pages File Format Full-Text
31704 44831 2010 8 PDF Available
Title
Metabolic engineering of Saccharomyces cerevisiae for the biotechnological production of succinic acid
Abstract

The production of bio-based succinic acid is receiving great attention, and several predominantly prokaryotic organisms have been evaluated for this purpose. In this study we report on the suitability of the highly acid- and osmotolerant yeast Saccharomyces cerevisiae as a succinic acid production host. We implemented a metabolic engineering strategy for the oxidative production of succinic acid in yeast by deletion of the genes SDH1, SDH2, IDH1 and IDP1. The engineered strains harbor a TCA cycle that is completely interrupted after the intermediates isocitrate and succinate. The strains show no serious growth constraints on glucose. In glucose-grown shake flask cultures, the quadruple deletion strain Δsdh1Δsdh2Δidh1Δidp1 produces succinic acid at a titer of 3.62 g L−1 (factor 4.8 compared to wild-type) at a yield of 0.11 mol (mol glucose)−1. Succinic acid is not accumulated intracellularly. This makes the yeast S. cerevisiae a suitable and promising candidate for the biotechnological production of succinic acid on an industrial scale.

Keywords
Yeast; Saccharomyces cerevisiae; Succinic acid; Metabolic engineering; Production
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Metabolic engineering of Saccharomyces cerevisiae for the biotechnological production of succinic acid
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Publisher
Database: Elsevier - ScienceDirect
Journal: Metabolic Engineering - Volume 12, Issue 6, November 2010, Pages 518–525
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us