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l-ascorbic acid producing yeasts learn from plants how to recycle it

Paper ID Volume ID Publish Year Pages File Format Full-Text
31746 44835 2011 9 PDF Available
Title
l-ascorbic acid producing yeasts learn from plants how to recycle it
Abstract

Microorganisms employed in industrial fermentation processes are often subjected to a variety of stresses that negatively affect growth, production and productivity. Therefore, stress robustness is an important property for their application. Reactive Oxygen Species (ROS) accumulation is a common denominator to a lot of these stress factors. Ascorbic acid (l-AA) acts as ROS scavenger, thus potentially protecting cells from harmful oxidative products. We have previously reported the development of Saccharomyces cerevisiae strains able to produce l-AA. This was obtained by expressing the known plant pathway genes and by complementing the missing step with an animal activity. Here, we show that l-AA accumulation inside yeast cells can be improved by expressing the complete biosynthetic plant pathway and, even further, by recycling its oxidation products.These new strains can be seen in a double perspective of exploitation: as novel organisms for vitamin C production and as novel cell factories for industrial processes.

Keywords
Saccharomyces cerevisiae; l-AA production; l-AA recycle; DHAR; Stress tolerancel-AA, l-ascorbic acid; EAA, eritro ascorbic acid; ROS, reactive oxygen species; MDHA, monodehydroascorbate; DHA, dehydroascorbate; GSH, glutathione; EGTA, ethylene glycol tetra
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Publisher
Database: Elsevier - ScienceDirect
Journal: Metabolic Engineering - Volume 13, Issue 2, March 2011, Pages 177–185
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
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Any Questions? feel free to contact us