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Tandem mass spectrometry: A novel approach for metabolic flux analysis

Paper ID Volume ID Publish Year Pages File Format Full-Text
31751 44835 2011 9 PDF Available
Title
Tandem mass spectrometry: A novel approach for metabolic flux analysis
Abstract

The goal of metabolic flux analysis (MFA) is the accurate estimation of intracellular fluxes in metabolic networks. Here, we introduce a new method for MFA based on tandem mass spectrometry (MS) and stable-isotope tracer experiments. We demonstrate that tandem MS provides more labeling information than can be obtained from traditional full scan MS analysis and allows estimation of fluxes with better precision. We present a modeling framework that takes full advantage of the additional labeling information obtained from tandem MS for MFA. We show that tandem MS data can be computed for any network model, any compound and any tandem MS fragmentation using linear mapping of isotopomers. The inherent advantages of tandem MS were illustrated in two network models using simulated and literature data. Application of tandem MS increased the observability of the models and improved the precision of estimated fluxes by 2- to 5-fold compared to traditional MS analysis.

Keywords
Mass spectrometry; GC–MS/MS; Metabolism; Isotopomer; Stable isotope tracer; 13C labelingGC, gas chromatography; MS, mass spectrometry; MFA, metabolic flux analysis; CID, collision-induced dissociation
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Publisher
Database: Elsevier - ScienceDirect
Journal: Metabolic Engineering - Volume 13, Issue 2, March 2011, Pages 225–233
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
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Full-text PDF Download
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Any Questions? feel free to contact us