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Understanding plasmid effect on hyaluronic acid molecular weight produced by Streptococcus equi subsp. zooepidemicus

Paper ID Volume ID Publish Year Pages File Format Full-Text
31761 44836 2010 8 PDF Available
Title
Understanding plasmid effect on hyaluronic acid molecular weight produced by Streptococcus equi subsp. zooepidemicus
Abstract

Hyaluronic acid is a biopolymer with valuable applications in the pharmaceutical and cosmetic industries. Streptococcus equi subspecies zooepidemicus cells transformed with a nisin-inducible, empty plasmid control displayed higher molecular weight. This increase in molecular weight is independent of the nisin promoter or antibiotic resistance. Using 2D DIGE followed by mass spectrometry, we identified up-regulation of the last step in UDP-N-acetyl-glucosamine biosynthesis (GlmU) and down-regulation of the first step in peptidoglycan biosynthesis (MurA) as possible mechanism for the plasmid effect. Over-expression of GlmU to further increase activity had no effect on UDP-N-acetyl-glucosamine levels or molecular weight, while over-expression of MurA reduced UDP-N-acetyl-glucosamine levels and molecular weight. Global transcriptional analysis revealed that differential regulation of GlmU and MurA activity was not reflected in transcription levels. This results, suggest that regulation is at a translational or post-translational level. Differential expression of two clp proteases may explain this effect as well as the small but significant changes in transcription levels of nearly 300 genes.

Keywords
Hyaluronic acid; Streptococcus zooepidemicus; Plasmid effect; Proteomics; Polysaccharides; Molecular weight control
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Publisher
Database: Elsevier - ScienceDirect
Journal: Metabolic Engineering - Volume 12, Issue 1, January 2010, Pages 62–69
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us