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Engineering a platform for photosynthetic isoprene production in cyanobacteria, using Synechocystis as the model organism

Paper ID Volume ID Publish Year Pages File Format Full-Text
31762 44836 2010 10 PDF Available
Title
Engineering a platform for photosynthetic isoprene production in cyanobacteria, using Synechocystis as the model organism
Abstract

The concept of “photosynthetic biofuels” envisions application of a single organism, acting both as photo-catalyst and producer of ready-made fuel. This concept was applied upon genetic engineering of the cyanobacterium Synechocystis, conferring the ability to generate volatile isoprene hydrocarbons from CO2 and H2O. Heterologous expression of the Pueraria montana (kudzu) isoprene synthase (IspS) gene in Synechocystis enabled photosynthetic isoprene generation in these cyanobacteria. Codon-use optimization of the kudzu IspS gene improved expression of the isoprene synthase in Synechocystis. Use of the photosynthesis psbA2 promoter, to drive the expression of the IspS gene, resulted in a light-intensity-dependent isoprene synthase expression. Results showed that oxygenic photosynthesis can be re-directed to generate useful small volatile hydrocarbons, while consuming CO2, without a prior requirement for the harvesting, dewatering and processing of the respective biomass.

Keywords
Biofuel; Cyanobacteria; Isoprene; Photosynthesis; Renewable energy; SynechocystisDXP, deoxyxylulose-5-phosphate; HMBPP, hydroxy-2-methyl-2-butenyl-4-diphosphate; G3P, glyceraldehyde-3-phosphate; Isp, isoprene; IspS, isoprene synthase; MEP, methyl-erythito
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Engineering a platform for photosynthetic isoprene production in cyanobacteria, using Synechocystis as the model organism
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Publisher
Database: Elsevier - ScienceDirect
Journal: Metabolic Engineering - Volume 12, Issue 1, January 2010, Pages 70–79
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us