fulltext.study @t Gmail

Metabolic engineering of Escherichia coli for α-farnesene production

Paper ID Volume ID Publish Year Pages File Format Full-Text
31766 44837 2011 8 PDF Available
Title
Metabolic engineering of Escherichia coli for α-farnesene production
Abstract

Sesquiterpenes are important materials in pharmaceuticals and industry. Metabolic engineering has been successfully used to produce these valuable compounds in microbial hosts. However, the microbial potential of sesquiterpene production is limited by the poor heterologous expression of plant sesquiterpene synthases and the deficient FPP precursor supply. In this study, we engineered E. coli to produce α-farnesene using a codon-optimized α-farnesene synthase and an exogenous MVA pathway. Codon optimization of α-farnesene synthase improved both the synthase expression and α-farnesene production. Augmentation of the metabolic flux for FPP synthesis conferred a 1.6- to 48.0-fold increase in α-farnesene production. An additional increase in α-farnesene production was achieved by the protein fusion of FPP synthase and α-farnesene synthase. The engineered E. coli strain was able to produce 380.0 mg/L of α-farnesene, which is an approximately 317-fold increase over the initial production of 1.2 mg/L.

► Codon optimization of α-farnesene synthase improved α-farnesene production. ► Insufficient supply of IPP and DMAPP limited α-farnesene production. ► Augmentation of metabolic flux for FPP synthesis increased α-farnesene production. ► FPP flux was directed to α-farnesene synthesis by protein fusion. ► Intermediates accumulation limited further increase of α-farnesene production.

Keywords
α-Farnesene; FPP synthesis; Mevalonate pathway; Codon optimization; Protein fusion
First Page Preview
Metabolic engineering of Escherichia coli for α-farnesene production
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us
Publisher
Database: Elsevier - ScienceDirect
Journal: Metabolic Engineering - Volume 13, Issue 6, November 2011, Pages 648–655
Authors
, , , , , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us