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Mapping photoautotrophic metabolism with isotopically nonstationary 13C flux analysis

Paper ID Volume ID Publish Year Pages File Format Full-Text
31767 44837 2011 10 PDF Available
Title
Mapping photoautotrophic metabolism with isotopically nonstationary 13C flux analysis
Abstract

Understanding in vivo regulation of photoautotrophic metabolism is important for identifying strategies to improve photosynthetic efficiency or re-route carbon fluxes to desirable end products. We have developed an approach to reconstruct comprehensive flux maps of photoautotrophic metabolism by computational analysis of dynamic isotope labeling measurements and have applied it to determine metabolic pathway fluxes in the cyanobacterium Synechocystis sp. PCC6803. Comparison to a theoretically predicted flux map revealed inefficiencies in photosynthesis due to oxidative pentose phosphate pathway and malic enzyme activity, despite negligible photorespiration. This approach has potential to fill important gaps in our understanding of how carbon and energy flows are systemically regulated in cyanobacteria, plants, and algae.

► Metabolic flux analysis was applied to a photoautotroph for the first time. ► Inefficiencies due to oxidative pentose phosphate and malic enzyme fluxes were identified. ► Photorespiration fluxes were negligible under standard growth conditions. ► This approach can be applied to map fluxes in autotrophic bacteria, microalgae, and plants.

Keywords
2PG, 2-phosphoglycolate; 2PGA, 2-phosphoglycerate; 3PGA, 3-phosphoglycerate; 6PGD, 6-phosphogluconate dehydrogenase; ACA, acetyl-CoA; AGT, alanine/glyoxylate aminotransferase; AKG, alpha-ketoglutarate; CIT, citrate; CS, citrate synthase; DHAP, dihydroxyac
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Mapping photoautotrophic metabolism with isotopically nonstationary 13C flux analysis
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Publisher
Database: Elsevier - ScienceDirect
Journal: Metabolic Engineering - Volume 13, Issue 6, November 2011, Pages 656–665
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us