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Manipulating respiratory levels in Escherichia coli for aerobic formation of reduced chemical products

Paper ID Volume ID Publish Year Pages File Format Full-Text
31772 44837 2011 9 PDF Available
Title
Manipulating respiratory levels in Escherichia coli for aerobic formation of reduced chemical products
Abstract

Optimizing the productivity of bioengineered strains requires balancing ATP generation and carbon atom conservation through fine-tuning cell respiration and metabolism. Traditional approaches manipulate cell respiration by altering air feeding, which are technically difficult especially in large bioreactors. An approach based on genetic regulation may better serve this purpose. With excess oxygen supply to the culture, we efficiently manipulated Escherichia coli cell respiration by adding different amount of coenzyme Q1 to strains lacking the ubiCA genes, which encode two critical enzymes for ubiquinone synthesis. As a proof-of-concept, the metabolic effect of the ubiCA gene knockout and coenzyme Q1 supplementation were characterized, and the metabolic profiles of the experimental strains showed clear correlations with coenzyme Q1 concentrations. Further proof-of-principle experiments were performed to illustrate that the approach can be used to optimize cell respiration for the production of chemicals of interest such as ethanol. This study showed that controlled respiration through genetic manipulation can be exploited to allow much larger operating windows for reduced product formation even under fully aerobic conditions.

► Manipulated Escherichia coli cell respiration. ► Novel strategy to increase metabolically engineered product formation through “controlled” respiration. ► Manipulating ubiquinone level in E. coli can be used to control cell metabolism.

Keywords
Escherichia coli; “Controlled respiration”; Ubiquinone; Metabolic distribution; Reduced product formation
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Manipulating respiratory levels in Escherichia coli for aerobic formation of reduced chemical products
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Publisher
Database: Elsevier - ScienceDirect
Journal: Metabolic Engineering - Volume 13, Issue 6, November 2011, Pages 704–712
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us