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Metabolic engineering of the complete pathway leading to heterologous biosynthesis of various flavonoids and stilbenoids in Saccharomyces cerevisiae

Paper ID Volume ID Publish Year Pages File Format Full-Text
31796 44839 2009 12 PDF Available
Title
Metabolic engineering of the complete pathway leading to heterologous biosynthesis of various flavonoids and stilbenoids in Saccharomyces cerevisiae
Abstract

Chemical or biological synthesis of plant secondary metabolites has attracted increasing interest due to their proven or assumed beneficial properties and health promoting effects. Resveratrol, a stilbenoid, naringenin, a flavanone, genistein, an isoflavone, and the flavonols kaempferol and quercetin have been shown to possess high nutritional and agricultural value. Four metabolically engineered yeast strains harboring plasmids with heterologous genes for enzymes involved in the biosynthesis of these compounds from phenylalanine have been constructed. Time course analyses of precursor utilization and end-product accumulation were carried out establishing the production of 0.29–0.31 mg/L of trans-resveratrol, 8.9–15.6 mg/L of naringenin, 0.1–7.7 mg/L of genistein, 0.9–4.6 mg/L of kaempferol and 0.26–0.38 mg/L of quercetin in defined media under optimal growth conditions. The recombinant yeast strains can be used further for the construction of improved flavonoid- and stilbenoid-overproducers.

Keywords
Flavonoids; Isoflavonoids; Stilbenoids; Metabolic engineering; Metabolic reconstitution; Plant biosynthesis; Genistein; Kaempferol; Naringenin; Quercetin; ResveratrolPAL, phenylalanine-ammonia lyase; C4H, cinnamic acid 4-hydroxylase; 4CL, 4-coumaric acid:
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Metabolic engineering of the complete pathway leading to heterologous biosynthesis of various flavonoids and stilbenoids in Saccharomyces cerevisiae
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Publisher
Database: Elsevier - ScienceDirect
Journal: Metabolic Engineering - Volume 11, Issue 6, November 2009, Pages 355–366
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us