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Rational design and construction of an efficient E. coli for production of diapolycopendioic acid

Paper ID Volume ID Publish Year Pages File Format Full-Text
31806 44840 2010 11 PDF Available
Title
Rational design and construction of an efficient E. coli for production of diapolycopendioic acid
Abstract

We applied elementary mode analysis to a recombinant metabolic network of carotenoid-producing E. coli in order to identify multiple-gene knockouts for an enhanced synthesis of the carotenoids diapolycopendial (DPL) and diapolycopendioic acid (DPA). Based on the model, all inefficient carotenoid biosynthesis pathways were eliminated in a strain containing a combination of eight gene deletions. To validate the model prediction, the designed strain was constructed and tested for its performance. The designed mutant produces the carotenoids at significantly increased yields and rates as compared to the wild-type. The consistency between model prediction and experimental results demonstrates that elementary mode analysis is useful as a guiding tool also for the rational strain design of more complex pathways for secondary metabolite production.

Keywords
Elementary mode analysis; Metabolic network analysis; Inverse metabolic engineering; carotenoid production; Escherichia coli metabolism
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Rational design and construction of an efficient E. coli for production of diapolycopendioic acid
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Publisher
Database: Elsevier - ScienceDirect
Journal: Metabolic Engineering - Volume 12, Issue 2, March 2010, Pages 112–122
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us