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Metabolic flux analysis in mammalian cell culture

Paper ID Volume ID Publish Year Pages File Format Full-Text
31811 44840 2010 11 PDF Available
Title
Metabolic flux analysis in mammalian cell culture
Abstract

Mammalian cell culture metabolism is characterized by glucoglutaminolysis, that is, high glucose and glutamine uptake combined with a high rate of lactate and non-essential amino acid secretion. Stress associated with acid neutralization and ammonia accumulation necessitates complex feeding schemes and limits cell densities achieved in fed-batch culture. Conventional and constraint-based metabolic flux analysis has been successfully used to study the metabolic phenotype of mammalian cells in culture, while 13C tracer analysis has been used to study small network models and validate assumptions of metabolism. Large-scale 13C metabolic flux analysis, which is required to improve confidence in the network models and their predictions, remains a major challenge. Advances in both modeling and analytical techniques are bringing this challenge within sight.

Keywords
Metabolic flux analysis (MFA); Mammalian cells; Review; Constrained-based analysis; Hybridoma; CHO
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Publisher
Database: Elsevier - ScienceDirect
Journal: Metabolic Engineering - Volume 12, Issue 2, March 2010, Pages 161–171
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us