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Design, construction and performance of the most efficient biomass producing E. coli bacterium

Paper ID Volume ID Publish Year Pages File Format Full-Text
31822 44841 2006 11 PDF Available
Title
Design, construction and performance of the most efficient biomass producing E. coli bacterium
Abstract

Inverse metabolic engineering based on elementary mode analysis was applied to maximize the biomass yield of Escherchia coli MG1655. Elementary mode analysis was previously employed to identify among 1691 possible pathways for cell growth the most efficient pathway with maximum biomass yield. The metabolic network analysis predicted that deletion of only 6 genes reduces the number of possible elementary modes to the most efficient pathway. We have constructed a strain containing these gene deletions and we evaluated its properties in batch and in chemostat growth experiments. The results show that the theoretical predictions are closely matched by the properties of the designed strain.

Keywords
Elementary (flux) mode; Metabolic network analysis; Inverse metabolic engineering; Biomass yield optimization
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Design, construction and performance of the most efficient biomass producing E. coli bacterium
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Publisher
Database: Elsevier - ScienceDirect
Journal: Metabolic Engineering - Volume 8, Issue 6, November 2006, Pages 628–638
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us