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Biosynthesis of poly(3-hydroxydecanoate) and 3-hydroxydodecanoate dominating polyhydroxyalkanoates by β-oxidation pathway inhibited Pseudomonas putida

Paper ID Volume ID Publish Year Pages File Format Full-Text
31830 44842 2011 7 PDF Available
Title
Biosynthesis of poly(3-hydroxydecanoate) and 3-hydroxydodecanoate dominating polyhydroxyalkanoates by β-oxidation pathway inhibited Pseudomonas putida
Abstract

Pseudomonas putida KT2442 produces medium-chain-length polyhydroxyalkanoates consisting of 3-hydroxyhexanoate (3HHx), 3-hydroxyoctanoate (3HO), 3-hydroxydecanoate (3HD), 3-hydroxydodecanoate (3HDD) and 3-hydroxytetradecanoate (3HTD) from relevant fatty acids. P. puitda KT2442 was found to contain key fatty acid degradation enzymes encoded by genes PP2136, PP2137 (fadB and fadA) and PP2214, PP2215 (fadB2x and fadAx), respectively. In this study, the above enzymes and other important fatty acid degradation enzymes, including 3-hydroxyacyl-CoA dehydrogenase and acyl-CoA dehydrogenase encoded by genes PP2047 and PP2048, respectively, were studied for their effects on PHA structures. Mutant P. puitda KTQQ20 was constructed by knocking out the above six genes and also 3-hydroxyacyl-CoA-acyl carrier protein transferase encoded by PhaG, leading to a significant reduction of fatty acid β-oxidation activity. Therefore, P. puitda KTQQ20 synthesized homopolymer poly-3-hydroxydecanoate (PHD) or P(3HD-co-84 mol% 3HDD), when grown on decanoic acid or dodecanoic acid. Melting temperatures of PHD and P(3HD-co-84 mol% 3HDD) were 72 and 78 °C, respectively. Thermal and mechanical properties of PHD and P(3HD-co-84 mol% 3HDD) were much better as compared with an mcl-PHA, consisting of lower content of C10 or C12 monomers. For the first time, it was shown that homopolymer PHD and 3HDD monomers dominating PHA could be synthesized by β-oxidation inhibiting P. putida grown on relevant carbon sources.

Keywords
PHB; Polyhydroxyalkanoates; Polyhydroxydecanoate; Metabolic engineering; β-oxidation; Pseudomonas putida
First Page Preview
Biosynthesis of poly(3-hydroxydecanoate) and 3-hydroxydodecanoate dominating polyhydroxyalkanoates by β-oxidation pathway inhibited Pseudomonas putida
Publisher
Database: Elsevier - ScienceDirect
Journal: Metabolic Engineering - Volume 13, Issue 1, January 2011, Pages 11–17
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering