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Central metabolic fluxes in the endosperm of developing maize seeds and their implications for metabolic engineering ☆

Paper ID Volume ID Publish Year Pages File Format Full-Text
31839 44842 2011 12 PDF Available
Title
Central metabolic fluxes in the endosperm of developing maize seeds and their implications for metabolic engineering ☆
Abstract

14C labeling experiments performed with kernel cultures showed that developing maize endosperm is more efficient than other non-photosynthetic tissues such as sunflower and maize embryos at converting maternally supplied substrates into biomass. To characterize the metabolic fluxes in endosperm, maize kernels were labeled to isotopic steady state using 13C-labeled glucose. The resultant labeling in free metabolites and biomass was analyzed by NMR and GC–MS. After taking into account the labeling of substrates supplied by the metabolically active cob, the fluxes through central metabolism were quantified by computer-aided modeling. The flux map indicates that 51–69% of the ATP produced is used for biomass synthesis and up to 47% is expended in substrate cycling. These findings point to potential engineering targets for improving yield and increasing oil contents by, respectively, reducing substrate cycling and increasing the commitment of plastidic carbon into fatty acid synthesis at the level of pyruvate kinase.

Keywords
Zea mays; Metabolic flux analysis; Carbon conversion efficiency; Endosperm assimilates; Futile cycling; Metabolic engineeringCCE, carbon conversion efficiency; DAP, days after pollination; GC–MS, gas chromatography–mass spectrometry; MFA, metabolic flux a
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Publisher
Database: Elsevier - ScienceDirect
Journal: Metabolic Engineering - Volume 13, Issue 1, January 2011, Pages 96–107
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
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Price was $35.95
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Price after discount Only $4.95
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