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Selection of objective function in genome scale flux balance analysis for process feed development in antibiotic production

Paper ID Volume ID Publish Year Pages File Format Full-Text
31854 44844 2008 7 PDF Available
Title
Selection of objective function in genome scale flux balance analysis for process feed development in antibiotic production
Abstract

Using flux variability analysis of a genome scale metabolic network of Streptomyces coelicolor, a series of reactions were identified, from disparate pathways that could be combined into an actinorhodin-generating mini-network. Candidate process feed nutrients that might be expected to influence this network were used in process simulations and in silico predictions compared to experimental findings. Ranking potential process feeds by flux balance analysis optimisation, using either growth or antibiotic production as objective function, did not correlate with experimental actinorhodin yields in fed processes. However, the effect of the feeds on glucose assimilation rate (using glucose uptake as objective function) ranked them in the same order as in vivo antibiotic production efficiency, consistent with results of a robustness analysis of the effect of glucose assimilation on actinorhodin production.

Keywords
Process feed design; Metabolic flux variability analysis; Streptomyces coelicolor; Genome scale
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Selection of objective function in genome scale flux balance analysis for process feed development in antibiotic production
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Publisher
Database: Elsevier - ScienceDirect
Journal: Metabolic Engineering - Volume 10, Issue 5, September 2008, Pages 227–233
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
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Full-text PDF Download
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