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Production of the polyketide 6-MSA in yeast engineered for increased malonyl-CoA supply

Paper ID Volume ID Publish Year Pages File Format Full-Text
31856 44844 2008 9 PDF Available
Title
Production of the polyketide 6-MSA in yeast engineered for increased malonyl-CoA supply
Abstract

The heterologous production of fungal polyketides was investigated using 6-methylsalicylic acid synthase (6-MSAS) as a model polyketide synthase and Saccharomyces cerevisiae as a host. In order to improve the production of 6-MSA by enhancing the supply of precursors, the promoter of the gene (ACC1) encoding acetyl-CoA carboxylase, which catalyzes the conversion of acetyl-CoA to malonyl-CoA, was replaced with a strong, constitutive promoter (TEF1p) in a strain harboring two plasmids carrying the genes encoding 6-MSAS from Penicillium patulum and PPTase from Aspergillus nidulans, respectively. The strain was characterized in batch cultivations with a glucose minimal media (20 g/L), and a 60% increase in 6-MSA titer was observed compared to a strain having the native promoter in front of ACC1. The production of 6-MSA was scaled up by the cultivation in minimal media containing 50 g/L of glucose, and hereby a final titer of 554±26 mg/L of 6-MSA was obtained.

Keywords
Acetyl-CoA carboxylase; Malonyl-CoA; Polyketides; Heterologous expression; Precursor supply
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Production of the polyketide 6-MSA in yeast engineered for increased malonyl-CoA supply
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Publisher
Database: Elsevier - ScienceDirect
Journal: Metabolic Engineering - Volume 10, Issue 5, September 2008, Pages 246–254
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us