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Ethylene production by metabolic engineering of the yeast Saccharomyces cerevisiae

Paper ID Volume ID Publish Year Pages File Format Full-Text
31859 44844 2008 5 PDF Available
Title
Ethylene production by metabolic engineering of the yeast Saccharomyces cerevisiae
Abstract

The non-ethylene producing yeast, Saccharomyces cerevisiae, was transformed into an ethylene producer by introducing the ethylene forming enzyme from the plant pathogenic bacterium Pseudomonas syringae. Cultivation of the metabolically engineered strain was performed in well-controlled bioreactors as aerobic batch cultures with an on-line monitoring of ethylene production. The highest productivity was obtained during the respiro-fermentative growth on glucose but there was also a significant rate of formation during the subsequent phase of ethanol respiration. Furthermore, investigations were performed whether substitution of the original nitrogen source, NH4+, for glutamate could improve productivity and yield of ethylene even more. The rationale being that one of the substrates for the enzyme is 2-oxoglutarate and this compound can be formed from glutamate in a single reaction. Indeed, there was a substantial improvement in the rate of production and the final yield of ethylene was almost three times higher when NH4+ was replaced by glutamate.

Keywords
Yeast; Saccharomyces; Ethylene; Bioreactor; Genetic modification
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Ethylene production by metabolic engineering of the yeast Saccharomyces cerevisiae
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Publisher
Database: Elsevier - ScienceDirect
Journal: Metabolic Engineering - Volume 10, Issue 5, September 2008, Pages 276–280
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
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