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Simultaneous detoxification, saccharification and co-fermentation of oil palm empty fruit bunch hydrolysate for l-lactic acid production by Bacillus coagulans JI12

Paper ID Volume ID Publish Year Pages File Format Full-Text
3186 156 2014 6 PDF Available
Title
Simultaneous detoxification, saccharification and co-fermentation of oil palm empty fruit bunch hydrolysate for l-lactic acid production by Bacillus coagulans JI12
Abstract

•Native detoxification of furfural by Bacillus coagulans JI12.•Simultaneous detoxification, saccharification & co-fermentation of lignocellulose.•Fermentation at 50 °C and pH 5.5 without sterilizing the medium.•Lactic acid productivity was as high as 3.4 g/L/h.

Bacillus coagulans JI12 was used to produce l-lactic acid from both cellulose and hemicellulose sugars of oil palm empty fruit bunch hydrolysate at 50 °C without sterilization prior to fermentation. In fermentation of mixed glucose and xylose (10 g/L:100 g/L or 50 g/L:53 g/L), both sugars were simultaneously converted to l-lactic acid. B. coagulans JI12 was tolerant against up to 4 g/L of furfural and 20 g/L of acetate and able to metabolize furfural to 2-furoic acid. After acid hydrolysis, both the hemicellulosic and cellulosic fractions of oil palm empty fruit bunch were fermented to lactic acid in a simultaneous detoxification, saccharification and co-fermentation process supplemented with 25 FPU Cellic® CTec2 cellulase per g cellulose, yielding 80.6 g/L of lactic acid with a productivity of 3.4 g/L/h. Neither pre-detoxification nor separation of fermentable sugars from lignin was required. These results indicate that B. coagulans JI12 is a promising strain for l-lactic acid production from lignocellulosic biomass.

Keywords
Simultaneous saccharification and co-fermentation; Detoxification; Bioprocess design; Cellulose; Lactic acid; ThermophilesEFB, empty fruit bunch; SSF, simultaneous saccharification and fermentation; SDSCF, simultaneous detoxification, saccharification and
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Simultaneous detoxification, saccharification and co-fermentation of oil palm empty fruit bunch hydrolysate for l-lactic acid production by Bacillus coagulans JI12
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 83, 15 February 2014, Pages 16–21
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us