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Improving production of bioactive secondary metabolites in actinomycetes by metabolic engineering

Paper ID Volume ID Publish Year Pages File Format Full-Text
31860 44844 2008 12 PDF Available
Title
Improving production of bioactive secondary metabolites in actinomycetes by metabolic engineering
Abstract

Production of secondary metabolites is a process influenced by several physico-chemical factors including nutrient supply, oxygenation, temperature and pH. These factors have been traditionally controlled and optimized in industrial fermentations in order to enhance metabolite production. In addition, traditional mutagenesis programs have been used by the pharmaceutical industry for strain and production yield improvement. In the last years, the development of recombinant DNA technology has provided new tools for approaching yields improvement by means of genetic manipulation of biosynthetic pathways. These efforts are usually focused in redirecting precursor metabolic fluxes, deregulation of biosynthetic pathways and overexpression of specific enzymes involved in metabolic bottlenecks. In addition, efforts have been made for the heterologous expression of biosynthetic gene clusters in other organisms, looking not only for an increase of production levels but also to speed the process by using rapidly growing and easy to manipulate organisms compared to the producing organism. In this review, we will focus on these genetic approaches as applied to bioactive secondary metabolites produced by actinomycetes.

Keywords
Streptomyces; Metabolism; Antibiotic biosynthesis; Precursor engineering; Heterologous expression; Regulation; Ribosome engineering; Genome shuffling
First Page Preview
Improving production of bioactive secondary metabolites in actinomycetes by metabolic engineering
Publisher
Database: Elsevier - ScienceDirect
Journal: Metabolic Engineering - Volume 10, Issue 5, September 2008, Pages 281–292
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering