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Expression of spearmint limonene synthase in transgenic spike lavender results in an altered monoterpene composition in developing leaves

Paper ID Volume ID Publish Year Pages File Format Full-Text
31865 44846 2008 12 PDF Available
Title
Expression of spearmint limonene synthase in transgenic spike lavender results in an altered monoterpene composition in developing leaves
Abstract

We generated transgenic spike lavender (Lavandula latifolia) plants constitutively expressing the limonene synthase (LS) gene from spearmint (Mentha spicata), encoding the LS enzyme that catalyzes the synthesis of limonene from geranyl diphosphate. Overexpression of the LS transgene did not consistently affect monoterpene profile in pooled leaves or flowers from transgenic T0 plants. Analyses from cohorts of leaves sampled at different developmental stages showed that essential oil accumulation in transgenic and control plants was higher in developing than in mature leaves. Furthermore, developing leaves of transgenic plants contained increased limonene contents (more than 450% increase compared to controls) that correlated with the highest transcript accumulation of the LS gene. The levels of other monoterpene pathway components were also significantly altered. T0 transgenic plants were grown for 2 years, self-pollinated, and the T1 seeds obtained. The increased limonene phenotype was maintained in the progenies that inherited the LS transgene.

Keywords
Essential oils; Lavandula latifolia; Limonene synthase; Metabolic engineering; Spike lavender; Monoterpenes
First Page Preview
Expression of spearmint limonene synthase in transgenic spike lavender results in an altered monoterpene composition in developing leaves
Publisher
Database: Elsevier - ScienceDirect
Journal: Metabolic Engineering - Volume 10, Issues 3–4, May–July 2008, Pages 166–177
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering