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Biosynthesis of CGTase by immobilized alkalophilic bacilli and crystallization of beta-cyclodextrin: Effective techniques to investigate cell immobilization and the production of cyclodextrins

Paper ID Volume ID Publish Year Pages File Format Full-Text
3187 156 2014 11 PDF Available
Title
Biosynthesis of CGTase by immobilized alkalophilic bacilli and crystallization of beta-cyclodextrin: Effective techniques to investigate cell immobilization and the production of cyclodextrins
Abstract

•Immobilized cells on loofa sponge showed operational stability for CGTase production.•Immobilized cells guided the relative formation of the β-cyclodextrin.•Purity of 89.5% was obtained for the crystallization of the β-cyclodextrin.•Tilden–Hudson technique was efficient for monitoring the cyclodextrin production.•FTIR-ATR suggested the interaction between Bacillus and fiber sponge by adsorption.

Cyclodextrin glycosyltransferase (CGTase) catalyses the degradation of starch, producing α-, β-, and γ-cyclodextrins (CDs). Immobilized cells offer several advantages, such as their prolonged and repeated use, ease separation from the fermentation medium, and reduced risk of contamination. The biosynthesis of CGTase and β-CD was optimized by immobilization of Bacillus firmus strain 37 and Bacillus sphaericus strain 41 cells on a loofa sponge. Both microorganisms produced significant levels of CGTase for three consecutive cycles (94.2 U/mL on average), increased the relative formation of the β-CD, and inhibited the formation of other CDs. The crystallization of the CD produced by both bacterial strains, when corn starch was used as the substrate, resulted in a 64% recovery of β-CD. The purity of the β-CD was 89.5% when maltodextrin was used as the substrate for B. firmus strain 37. The Tilden–Hudson technique was used as a simple, inexpensive, and efficient method for monitoring the continuous production of CDs using immobilized cells. The innovative use of the Fourier Transform Infrared Spectroscopy technique by means of the Attenuated Total Reflectance method suggested that the interaction between the B. sphaericus strain 41 cells and the loofa sponge occurred by natural adsorption.

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Keywords
Cyclodextrins; CGTase; Biosynthesis; Enzyme production; Immobilized cells; Purification
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Biosynthesis of CGTase by immobilized alkalophilic bacilli and crystallization of beta-cyclodextrin: Effective techniques to investigate cell immobilization and the production of cyclodextrins
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 83, 15 February 2014, Pages 22–32
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us