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Metabolomics for high-resolution monitoring of the cellular physiological state in cell culture engineering

Paper ID Volume ID Publish Year Pages File Format Full-Text
31873 44847 2010 11 PDF Available
Title
Metabolomics for high-resolution monitoring of the cellular physiological state in cell culture engineering
Abstract

Cell culture engineering has to-date used transcriptomic, proteomic, and metabolic flux analyses, attempting to resolve significant questions regarding cell culture performance. Despite the foreseen positive impact, the metabolomic analytical platform has not yet been vastly deployed. Presently, there is no published application of metabolomics to industrial-scale mammalian cell culture. We applied gas chromatography–mass spectrometry metabolomics to analyze baby hamster kidney (BHK) cells of various cell ages cultivated in high-cell density perfusion reactors at both laboratory and manufacturing scales. Cell growth, metabolic activity and protein productivity measurements, which are currently used to monitor the cellular physiological state, suggested consistency across bioreactors and over the course of the cultivation. Nevertheless, metabolomic profiling enabled the differentiation of cell cultures based on cell age, bioreactor scale and cell source. These results support the usefulness of metabolomics as a high resolution molecular analysis tool in cell culture engineering.

Keywords
Bioprocess development and optimization of mammalian cell cultures; Therapeutic protein production; Gas chromatography–mass spectrometry (GC–MS) metabolic profiling; Perfusion system; High-throughput molecular analysis
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Publisher
Database: Elsevier - ScienceDirect
Journal: Metabolic Engineering - Volume 12, Issue 3, May 2010, Pages 212–222
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us