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Autonomous induction of recombinant proteins by minimally rewiring native quorum sensing regulon of E. coli

Paper ID Volume ID Publish Year Pages File Format Full-Text
31880 44847 2010 7 PDF Available
Title
Autonomous induction of recombinant proteins by minimally rewiring native quorum sensing regulon of E. coli
Abstract

Quorum sensing (QS) enables an individual bacterium's metabolic state to be communicated to and ultimately control the phenotype of an emerging population. Harnessing the hierarchical nature of this signal transduction process may enable the exploitation of individual cell characteristics to direct or “program” entire populations of cells. We re-engineered the native QS regulon so that individual cell signals (autoinducers) are used to guide high level expression of recombinant proteins in E. coli populations. Specifically, the autoinducer-2 (AI-2) QS signal initiates and guides the overexpression of green fluorescent protein (GFP), chloramphenicol acetyl transferase (CAT) and β-galactosidase (LacZ). The new process requires no supervision or input (e.g., sampling for optical density measurement, inducer addition, or medium exchange) and represents a low-cost, high-yield platform for recombinant protein production. Moreover, rewiring a native signal transduction circuit exemplifies an emerging class of metabolic engineering approaches that target regulatory functions.

Keywords
Quorum sensing; AI-2; Recombinant protein expression; Metabolic engineering; Metabolic burden; Systems biologyQS, quorum sensing; AI-2, autoinducer-2; Pfs, S-adenosylhomocysteine nucleosidase; LuxS, S-ribosylhomocysteinase; LsrK, LuxS responding AI-2 kina
First Page Preview
Autonomous induction of recombinant proteins by minimally rewiring native quorum sensing regulon of E. coli
Publisher
Database: Elsevier - ScienceDirect
Journal: Metabolic Engineering - Volume 12, Issue 3, May 2010, Pages 291–297
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering