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Metabolic engineering of Escherichia coli for 1-butanol and 1-propanol production via the keto-acid pathways

Paper ID Volume ID Publish Year Pages File Format Full-Text
31886 44849 2008 9 PDF Available
Title
Metabolic engineering of Escherichia coli for 1-butanol and 1-propanol production via the keto-acid pathways
Abstract

Production of higher alcohols via the keto-acid intermediates found in microorganism's native amino-acid pathways has recently shown promising results. In this work, an Escherichia coli strain that produces 1-butanol and 1-propanol from glucose was constructed. The strain first converts glucose to 2-ketobutyrate, a common keto-acid intermediate for isoleucine biosynthesis. Then, 2-ketobutyrate is converted to 1-propanol through reactions catalyzed by the heterologous decarboxylase and dehydrogenase, or to 1-butanol via the chemistry involved in the synthesis of the unnatural amino acid norvaline. We systematically improved the synthesis of 1-propanol and 1-butanol through deregulation of amino-acid biosynthesis and elimination of competing pathways. The final strain demonstrated a production titer of 2 g/L with nearly 1:1 ratio of butanol and propanol.

Keywords
Metabolic engineering; Biofuels; Alcohols; Butanol
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Metabolic engineering of Escherichia coli for 1-butanol and 1-propanol production via the keto-acid pathways
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Publisher
Database: Elsevier - ScienceDirect
Journal: Metabolic Engineering - Volume 10, Issue 6, November 2008, Pages 312–320
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
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Any Questions? feel free to contact us