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The Acinetobacter sp. chnB promoter together with its cognate positive regulator ChnR is an attractive new candidate for metabolic engineering applications in bacteria

Paper ID Volume ID Publish Year Pages File Format Full-Text
31909 44851 2008 9 PDF Available
Title
The Acinetobacter sp. chnB promoter together with its cognate positive regulator ChnR is an attractive new candidate for metabolic engineering applications in bacteria
Abstract

Over the last 10 years there has been an extremely fast development in the global characterization of bacteria at the genome, transcriptome, proteome and metabolome levels. To further explore and apply these complex data sets there is now a need for new biological tools that can be used to test or verify hypotheses generated on the basis of all the new information. Here, we report the integration of an expression cassette based on the Acinetobacter sp. chnB promoter and its cognate positive regulator chnR gene into a replicon derived from the broad-host-range plasmid RK2. Cyclohexanone was found to be the most efficient inducer of this system in Escherichia coli, using firefly luciferase as a reporter. To explore the potential of the system in another species, we show that the system can be used in combination with another similar expression cassette (Pm/xylS) to control the monomer composition of the industrially widely used exopolysaccharide alginate, produced by Pseudomonas fluorescens.

Keywords
Pm; XylS; ChnB; ChnR; Metabolic engineering; Promoter
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The Acinetobacter sp. chnB promoter together with its cognate positive regulator ChnR is an attractive new candidate for metabolic engineering applications in bacteria
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Publisher
Database: Elsevier - ScienceDirect
Journal: Metabolic Engineering - Volume 10, Issue 2, March 2008, Pages 121–129
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us