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A microrespirometric method for the determination of stoichiometric and kinetic parameters of heterotrophic and autotrophic cultures

Paper ID Volume ID Publish Year Pages File Format Full-Text
3192 156 2014 9 PDF Available
Title
A microrespirometric method for the determination of stoichiometric and kinetic parameters of heterotrophic and autotrophic cultures
Abstract

•A microrespirometry method was developed in a 24-well microreactor system.•The method was tested for the determination KS, μmax and YX/S of four cultures.•These parameters were determined with lower error than with standard respirometry.•This method is of interest for meaningful process developments in short times.

Respirometry, which consists in the measurement of the oxygen uptake rate, is a method commonly used to determine kinetic and stoichiometric parameters of biological processes. In the present work, a novel microrespironetry method, which involves standard respirometry procedures but in a microreactor array, was developed and assessed, based on a standard commercial apparatus. First, the volumetric oxygen transfer coefficient (KLa), which is important for the interpretation of respirometric data, was determined under an exhaustive set of experimental conditions. KLa ranged from 2.2 to 48.0 h−1, which is similar to KLa typically found in standard respirometers. Then, the method was assessed through the determination of the maximum specific growth rate, the half-saturation constant and the growth yield of axenic and mixed cultures of autotrophic and heterotrophic bacteria. The selected cultures were Pseudomonas putida F1, Nitrobacter winogradskyi, an activated sludge and a nitrifying consortium. Additionally to reporting parameters for these cultures, this report shows that the microrespirometry method allowed for the determination of stoichiometric and kinetic parameters. Simultaneous tests done with a single culture sample, made possible by the microreactor array, allowed parameters determination with a lower standard error than observed with traditional respirometry and with much less experimental effort.

Keywords
Microtiter plate; Microwell plate; Bioreactors; Mass transfer; Kinetic parameters; Dissolved oxygen
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A microrespirometric method for the determination of stoichiometric and kinetic parameters of heterotrophic and autotrophic cultures
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 83, 15 February 2014, Pages 70–78
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
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Any Questions? feel free to contact us