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Metabolic engineering of the E. colil-phenylalanine pathway for the production of d-phenylglycine (d-Phg)

Paper ID Volume ID Publish Year Pages File Format Full-Text
31922 44853 2006 13 PDF Available
Title
Metabolic engineering of the E. colil-phenylalanine pathway for the production of d-phenylglycine (d-Phg)
Abstract

d-phenylglycine (d-Phg) is an important side chain building block for semi-synthetic penicillins and cephalosporins such as ampicillin and cephalexin. To produce d-Phg ultimately from glucose, metabolic engineering was applied. Starting from phenylpyruvate, which is the direct precursor of l-phenylalanine, an artificial d-Phg biosynthesis pathway was created. This three-step route is composed of the enzymes hydroxymandelate synthase (HmaS), hydroxymandelate oxidase (Hmo), and the stereoinverting hydroxyphenylglycine aminotransferase (HpgAT). Together they catalyse the conversion of phenylpyruvate via mandelate and phenylglyoxylate to d-Phg. The corresponding genes were obtained from Amycolatopsis orientalis, Streptomyces coelicolor, and Pseudomonas putida. Combined expression of these activities in E. coli strains optimized for the production of l-phenylalanine resulted in the first completely fermentative production of d-Phg.

Keywords
Aromatic amino acids; d-Amino acids; Renewable; Sustainable; Fine chemicals
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Metabolic engineering of the E. colil-phenylalanine pathway for the production of d-phenylglycine (d-Phg)
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Publisher
Database: Elsevier - ScienceDirect
Journal: Metabolic Engineering - Volume 8, Issue 3, May 2006, Pages 196–208
Authors
, , , , , , , , , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us