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A comparative study of metabolic engineering anti-metabolite tolerance in Escherichia coli

Paper ID Volume ID Publish Year Pages File Format Full-Text
31924 44853 2006 13 PDF Available
Title
A comparative study of metabolic engineering anti-metabolite tolerance in Escherichia coli
Abstract

A problem in strain engineering is that mutations that benefit the expression of a phenotype in one environment may impose a cost to biological fitness in a new environment. The overall objective of this study was to improve understanding of this phenomenon within the context of a classic anti-metabolite selection strategy. We have engineered Escherichia coli using three mutagenesis techniques (chemical mutagenesis, insertional mutagenesis, and plasmid-based overexpression) and assessed the relative costs and benefits to biological fitness of mutants selected for tolerance to five amino acid analogs whose target amino acids (glutamatic acid, aspartic acid, tryptophan, glycine, and serine) differ in metabolic connectivity and biosynthetic energy requirements. Our major findings include (i) the fold increase in anti-metabolite tolerance, independent of mutagenesis strategy, was much greater for aspartic acid β-hydroxamate (AAH) compared to all other tested hydroxamates, (ii) increased tolerance to glutamic acid γ-hydroxamate (GAH) was not achieved using any of the mutagenesis strategies, and (iii) characteristics of the anti-metabolite, rather than those of the corresponding metabolite, were more important in determining the ability to increase tolerance.

Keywords
Metabolic engineering; Chemical mutagenesis; Insertional mutagenesis; Gene overexpression; Amino acid analog; Escherichia coli
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A comparative study of metabolic engineering anti-metabolite tolerance in Escherichia coli
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Publisher
Database: Elsevier - ScienceDirect
Journal: Metabolic Engineering - Volume 8, Issue 3, May 2006, Pages 227–239
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us