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Enzyme immobilization by fouling in ultrafiltration membranes: Impact of membrane configuration and type on flux behavior and biocatalytic conversion efficacy

Paper ID Volume ID Publish Year Pages File Format Full-Text
3193 156 2014 11 PDF Available
Title
Enzyme immobilization by fouling in ultrafiltration membranes: Impact of membrane configuration and type on flux behavior and biocatalytic conversion efficacy
Abstract

•Membrane fouling formation can be regarded as an immobilization process.•Alcohol dehydrogenase is immobilized in membranes by simple filtration.•Support layer facing feed can increase enzyme loading.•The trade-off between permeate flux and enzyme loading can be minimized.•Regenerated cellulose membranes can retain enzyme activity for a long time.

Enzyme-immobilization in membranes accomplished by fostering membrane fouling was evaluated. Four different membrane configurations and five membranes were compared for immobilization of alcohol dehydrogenase (ADH) in terms of enzyme loading, permeate flux and final biocatalytic conversion. The membrane configuration impacted the efficiency of the enzyme-immobilization as well as the biocatalytic-membrane reaction, and the “sandwich mode”, with an extra polypropylene support above the membrane skin layer, worked best due to its high flux and stable conversion. Among the membranes, a GR51PP polysulphone membrane allowed for the highest flux during the reaction with the enzyme-immobilized membrane. At the same time, the lowest enzyme loading and low reaction stability were achieved for this membrane. Satisfactory enzyme loadings, stable conversions, but low flux rates were obtained for the PLTK and PLGC regenerated cellulose membranes. With these two highly hydrophilic membranes, the ADH enzyme activity was fully retained even after 24 h of storage of the membrane. Filtration blocking and resistance models were used to analyze the fouling/immobilization mechanisms and give explanations for the different results. The work confirms that fouling-induced enzyme immobilization is a promising option for enhancing biocatalytic productivity, and highlights the significance of the membrane type and configuration for optimal performance.

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Keywords
Membrane fouling; Enzyme immobilization; Alcohol dehydrogenase; Filtration; Catalysis
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Enzyme immobilization by fouling in ultrafiltration membranes: Impact of membrane configuration and type on flux behavior and biocatalytic conversion efficacy
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 83, 15 February 2014, Pages 79–89
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us