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Hydrolysis and fermentation of amorphous cellulose by recombinant Saccharomyces cerevisiae

Paper ID Volume ID Publish Year Pages File Format Full-Text
31953 44858 2007 8 PDF Available
Title
Hydrolysis and fermentation of amorphous cellulose by recombinant Saccharomyces cerevisiae
Abstract

In this study, we expressed two cellulase encoding genes, an endoglucanase of Trichoderma reesei (EGI) and the β-glucosidase of Saccharomycopsis fibuligera (BGL1), in combination in Saccharomyces cerevisiae. The resulting strain was able to grow on phosphoric acid swollen cellulose (PASC) through simultaneous production of sufficient extracellular endoglucanase and β-glucosidase activity. Anaerobic growth (0.03 h−1) up to 0.27 g l−1 DCW was observed on medium containing 10 g l−1 PASC as sole carbohydrate source with concomitant ethanol production of up to 1.0 g l−1. We have thus demonstrated the construction of a yeast strain capable of growth on and one-step conversion of amorphous cellulose to ethanol, representing significant progress towards realization of one-step processing of cellulosic biomass in a consolidated bioprocessing configuration. To our knowledge, this is the first report of a recombinant strain of S. cerevisiae growing on pure cellulose.

Keywords
Endoglucanase; β-Glucosidase; Cellulase expression in yeast; Consolidated bioprocessing
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Hydrolysis and fermentation of amorphous cellulose by recombinant Saccharomyces cerevisiae
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Publisher
Database: Elsevier - ScienceDirect
Journal: Metabolic Engineering - Volume 9, Issue 1, January 2007, Pages 87–94
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us