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Enzymic analysis of NADPH metabolism in β-lactam-producing Penicillium chrysogenum: Presence of a mitochondrial NADPH dehydrogenase

Paper ID Volume ID Publish Year Pages File Format Full-Text
31957 44861 2006 11 PDF Available
Title
Enzymic analysis of NADPH metabolism in β-lactam-producing Penicillium chrysogenum: Presence of a mitochondrial NADPH dehydrogenase
Abstract

Based on assumed reaction network structures, NADPH availability has been proposed to be a key constraint in β-lactam production by Penicillium chrysogenum. In this study, NADPH metabolism was investigated in glucose-limited chemostat cultures of an industrial P. chrysogenum strain. Enzyme assays confirmed the NADP+-specificity of the dehydrogenases of the pentose-phosphate pathway and the presence of NADP+-dependent isocitrate dehydrogenase. Pyruvate decarboxylase/NADP+-linked acetaldehyde dehydrogenase and NADP+-linked glyceraldehyde-3-phosphate dehydrogenase were not detected. Although the NADPH requirement of penicillin-G-producing chemostat cultures was calculated to be 1.4–1.6-fold higher than that of non-producing cultures, in vitro measured activities of the major NADPH-providing enzymes were the same. Isolated mitochondria showed high rates of antimycin A-sensitive respiration of NADPH, thus indicating the presence of a mitochondrial NADPH dehydrogenase that oxidises cytosolic NADPH. The presence of this enzyme in P. chrysogenum might have important implications for stoichiometric modelling of central carbon metabolism and β-lactam production and may provide an interesting target for metabolic engineering.

Keywords
Penicillium chrysogenum; Penicillin-G; NADPH; Redox metabolism; Chemostat cultures; Metabolic engineering; Respiratory chain; MitochondriaαAAA, α-aminoadipic acid, LLD-ACV, l-α-aminoadipyl-l-cysteinyl-d-valine; PAA, phenylacetic acid; rcf, rotational cent
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Enzymic analysis of NADPH metabolism in β-lactam-producing Penicillium chrysogenum: Presence of a mitochondrial NADPH dehydrogenase
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Publisher
Database: Elsevier - ScienceDirect
Journal: Metabolic Engineering - Volume 8, Issue 2, March 2006, Pages 91–101
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us