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In silico aided metabolic engineering of Saccharomyces cerevisiae for improved bioethanol production

Paper ID Volume ID Publish Year Pages File Format Full-Text
31958 44861 2006 10 PDF Available
Title
In silico aided metabolic engineering of Saccharomyces cerevisiae for improved bioethanol production
Abstract

In silico genome-scale cell models are promising tools for accelerating the design of cells with improved and desired properties. We demonstrated this by using a genome-scale reconstructed metabolic network of Saccharomyces cerevisiae to score a number of strategies for metabolic engineering of the redox metabolism that will lead to decreased glycerol and increased ethanol yields on glucose under anaerobic conditions. The best-scored strategies were predicted to completely eliminate formation of glycerol and increase ethanol yield with 10%. We successfully pursued one of the best strategies by expressing a non-phosphorylating, NADP+-dependent glyceraldehyde-3-phosphate dehydrogenase in S. cerevisiae. The resulting strain had a 40% lower glycerol yield on glucose while the ethanol yield increased with 3% without affecting the maximum specific growth rate. Similarly, expression of GAPN in a strain harbouring xylose reductase and xylitol dehydrogenase led to an improvement in ethanol yield by up to 25% on xylose/glucose mixtures.

Keywords
Saccharomyces cerevisiae; Genome-scale model; Redox metabolism; Non-phosphorylating NADP+-dependent glyceraldehydes-3-phosphate dehydrogenase; Ethanol; Glycerol; Xylose
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In silico aided metabolic engineering of Saccharomyces cerevisiae for improved bioethanol production
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Publisher
Database: Elsevier - ScienceDirect
Journal: Metabolic Engineering - Volume 8, Issue 2, March 2006, Pages 102–111
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us