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Links between metabolism and apoptosis in mammalian cells: Applications for anti-apoptosis engineering

Paper ID Volume ID Publish Year Pages File Format Full-Text
31966 44862 2007 10 PDF Available
Title
Links between metabolism and apoptosis in mammalian cells: Applications for anti-apoptosis engineering
Abstract

Production of complex recombinant proteins requires the culture of mammalian cells in bioreactors. Inherent in these cultures is the problem of cell death, which can result from nutrient depletion, byproduct accumulation, and other bioreactor stresses which signal the cell to die through apoptosis, or programmed cell death. Apoptosis is a highly regulated pathway of both pro- and anti-apoptotic proteins that promote cell survival or death, and cell engineering efforts to inhibit the apoptosis pathway have led to increased culture viability and recombinant protein production. Originally, the exclusive function of many of these pathway proteins was believed to be binding at the mitochondria and regulating apoptosis through modulation of the mitochondria permeability. While this protein functionality does still hold true, it is now evident that these proteins also include roles in the metabolic processes of the mitochondria. Furthermore, apoptosis pathway proteins in other organelles within the cell may also both modulate apoptosis and metabolism. This review first details the known links that exist between apoptosis proteins and metabolic functions in the cytosol, mitochondria, and endoplasmic reticulum. Second, the review turns to look at potentially new cell engineering strategies that are linked to metabolism for improving cell culture viability and protein production.

Keywords
Metabolism; Apoptosis; Bcl-2; Mammalian cell culture; Anti-apoptosis; Cell engineering; Mitochondria; Bcl-xL; Calcium; Glycolysis; Hexokinase; VDAC
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Links between metabolism and apoptosis in mammalian cells: Applications for anti-apoptosis engineering
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Publisher
Database: Elsevier - ScienceDirect
Journal: Metabolic Engineering - Volume 9, Issue 4, July 2007, Pages 317–326
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us