fulltext.study @t Gmail

Characteristics of methionine production by an engineered Corynebacterium glutamicum strain

Paper ID Volume ID Publish Year Pages File Format Full-Text
31967 44862 2007 10 PDF Available
Title
Characteristics of methionine production by an engineered Corynebacterium glutamicum strain
Abstract

A methionine-producing strain was derived from a lysine-producing Corynebacterium glutamicum through a process of genetic manipulation in order to assess its potential to synthesize and accumulate methionine during growth. The strain carries a deregulated hom gene (homFBR) to abolish feedback inhibition of homoserine dehydrogenase by threonine and a deletion of the thrB gene (ΔthrB) to abolish threonine synthesis. The constructed C. glutamicum MH20-22B/homFBR/ΔthrB strain accumulated 2.9 g/l of methionine by batch fermentation and showed resistance to methionine analogue ethionine at concentrations up to 30 mM. The growth of the strain was apparently impaired as a result of the accumulation of methionine biosynthetic intermediate, homocysteine. Production assays also revealed that the accumulation of methionine in the growth medium was transient and declined as the carbon source was depleted. During the period of methionine disappearance, the methionine biosynthetic genes were completely repressed in the engineered strains but not in the parental strain. After all, we have not only successfully constructed a methionine-producing C. glutamicum strain by genetic manipulation, but also revealed cellular constraints in attaining high yield and productivity.

Keywords
Corynebacterium glutamicum; Methionine
First Page Preview
Characteristics of methionine production by an engineered Corynebacterium glutamicum strain
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us
Publisher
Database: Elsevier - ScienceDirect
Journal: Metabolic Engineering - Volume 9, Issue 4, July 2007, Pages 327–336
Authors
, , , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us