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Global transcription machinery engineering: A new approach for improving cellular phenotype

Paper ID Volume ID Publish Year Pages File Format Full-Text
31987 44867 2007 10 PDF Available
Title
Global transcription machinery engineering: A new approach for improving cellular phenotype
Abstract

It is now generally accepted that most cellular phenotypes are affected by many genes. As a result, engineering a desired phenotype would be facilitated enormously by simultaneous multiple gene modification, yet the capacity to introduce such modifications is very limited. Here, we demonstrate that the components of global cellular transcription machinery (specifically, σ70) can be engineered to allow for global perturbations of the transcriptome, which can help unlock complex phenotypes. Results from three distinct phenotypes (ethanol tolerance, metabolite overproduction, and multiple phenotypes) are provided as proof-of-concept. In each case, the tool of global transcription machinery engineering (gTME) outperformed traditional approaches by quickly and more effectively optimizing phenotypes.

Keywords
Sigma factor engineering; Ethanol tolerance; Metabolic engineering; Directed evolution; Lycopene production
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Publisher
Database: Elsevier - ScienceDirect
Journal: Metabolic Engineering - Volume 9, Issue 3, May 2007, Pages 258–267
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us