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Functional expression of prokaryotic and eukaryotic genes in Escherichia coli   for conversion of glucose to pp-hydroxystyrene

Paper ID Volume ID Publish Year Pages File Format Full-Text
31988 44867 2007 9 PDF Available
Title
Functional expression of prokaryotic and eukaryotic genes in Escherichia coli   for conversion of glucose to pp-hydroxystyrene
Abstract

The chemical monomer pp-hydroxystyrene (pHS) is used for producing a number of important industrial polymers from petroleum-based feedstocks. In an alternative approach, the microbial production of pHS can be envisioned by linking together a number of different metabolic pathways, of which those based on using glucose for carbon and energy are currently the most economical. The biological process conserves petroleum when glucose is converted to the aromatic amino acid L-tyrosine, which is deaminated by a tyrosine/phenylalanine ammonia-lyase (PAL/TAL) enzyme to yield pp-hydroxycinnamic acid (pHCA). Subsequent decarboxylation of pHCA gives rise to pHS. Bacteria able to efficiently decarboxylate pHCA to pHS using a pHCA decarboxylase (PDC) include Bacillus subtilis, Pseudomonas fluorescens and Lactobacillus plantarum. Both B. subtilis and L. plantarum possess high levels of pHCA-inducible decarboxylase activity and were chosen for further studies. The genes encoding PDC in these organisms were cloned and the pHCA decarboxylase expressed in Escherichia coli strains co-transformed with a plasmid encoding a bifunctional PAL/TAL enzyme from the yeast Rhodotorula glutinis. Production of pHS from glucose was ten-fold greater for the expressed L. plantarum pdc gene (0.11 mM), compared to that obtained when the B. subtilis PDC gene (padC) was used. An E. coli strain (WWQ51.1) expressing both tyrosine ammonia-lyase(PAL) and pHCA decarboxylase (pdc), when grown in a 14 L fermentor and under phosphate limited conditions, produced 0.4 g/L of pHS from glucose. We, therefore, demonstrate pHS production from an inexpensive carbohydrate feedstock by fermentation using a novel metabolic pathway comprising genes from E. coli, L. plantarum and R. glutinis.

Keywords
Microbial production of pp-hydroxystyrene (4-vinyl phenol); Conversion of glucose to pp-hydroxystyrene; Metabolic engineering; pp-hydroxycinnamic acid decarboxylase; pp-coumarate decarboxylase; Phenylalanine ammonia-lyase; Tyrosine ammonia-lyase
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Functional expression of prokaryotic and eukaryotic genes in Escherichia coli   for conversion of glucose to pp-hydroxystyrene
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Publisher
Database: Elsevier - ScienceDirect
Journal: Metabolic Engineering - Volume 9, Issue 3, May 2007, Pages 268–276
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us