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Evolution of a Saccharomyces cerevisiae metabolic pathway in Escherichia coli

Paper ID Volume ID Publish Year Pages File Format Full-Text
31996 44872 2007 8 PDF Available
Title
Evolution of a Saccharomyces cerevisiae metabolic pathway in Escherichia coli
Abstract

The Saccharomyces cerevisiae glycerol pathway (GPD1 and GPP2) was evolved in vivo in Escherichia coli. The central metabolism of E. coli was engineered to link glucose consumption and glycerol production. The engineered strain was evolved in a chemostat culture and a high glycerol producer was rapidly obtained. The evolution of the strain was associated to a deletion between GPD1 and GPP2, resulting in the production of a fusion protein with both glycerol-3-P dehydrogenase and glycerol-3-P phosphatase activities. The higher efficiency of the fusion protein was due to partial glycerol-3-P channeling between the two active sites. The evolved strain produces glycerol from glucose at high yield, concentration and productivity.

Keywords
Metabolic pathway evolution; Central metabolism engineering; Genes fusion; Protein fusion
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Evolution of a Saccharomyces cerevisiae metabolic pathway in Escherichia coli
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Publisher
Database: Elsevier - ScienceDirect
Journal: Metabolic Engineering - Volume 9, Issue 2, March 2007, Pages 152–159
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us