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Signal transduction and metabolic flux of β-thujaplicin and monoterpene biosynthesis in elicited Cupressus lusitanica cell cultures

Paper ID Volume ID Publish Year Pages File Format Full-Text
32006 44875 2006 16 PDF Available
Title
Signal transduction and metabolic flux of β-thujaplicin and monoterpene biosynthesis in elicited Cupressus lusitanica cell cultures
Abstract

β-Thujaplicin is an antimicrobial tropolone derived from geranyl pyrophosphate(GPP) and monoterpene intermediate. Yeast elicitor-treated Cupressus lusitanica cell cultures accumulate high levels of β-thujaplicin at early stages and other monoterpenes at later stages post-elicitation. The different regulation of β-thujaplicin and monoterpene biosynthesis and signal transduction directing metabolic flux to β-thujaplicin firstly and then shifting metabolic flow from β-thujaplicin to other monoterpene biosynthesis were investigated. The earlier rapid induction of β-thujaplicin accumulation and a later stimulation of monoterpene biosynthesis by yeast elicitor are in well agreement with elicitor-induced changes in activity of three monoterpene biosynthetic enzymes including isopentenyl pyrophosphate isomerase, GPP synthase, and monoterpene synthase. Yeast elicitor induces an earlier and stronger β-thujaplicin production and monoterpene biosynthetic enzyme activity than methyl jasmonate (MeJA) does. Profiling all monoterpenes produced by C. lusitanica cell cultures under different conditions reveals that β-thujaplicin biosynthesis parallels with other monoterpenes and competes for common precursor pools. Yet β-thujaplicin is produced pre-dominantly at early stage of elicitation whereas other monoterpenes are mainly accumulated at late stage while β-thujaplicin is metabolized. It is suggested that yeast elicitor-treated C. lusitanica cells preferentially accumulate β-thujaplicin as a primary defense and other monoterpenes as a secondary defense. Inhibitor treatments suggest that immediate production of β-thujaplicin post-elicitation largely depends on pre-existing enzymes and translation of pre-existing transcripts as well as recruitment of precursor pools from both the cytosol and plastids. The later β-thujaplicin and other monoterpene accumulation strictly depends on active transcription and translation. Induction of β-thujaplicin production and activation of monoterpene biosynthetic enzymes by elicitor involves similar signaling pathways, which may activate early β-thujaplicin production and later monoterpene biosynthesis and induce a metabolic flux shift from β-thujaplicin to monoterpene accumulation.

Keywords
Biosynthetic enzyme; Cupressus lusitanica cell culture; Metabolic flux; Monoterpene profile; Signal transduction; β-thujaplicinAD, Actinomycin D; CH, cycloheximide; DMAPP, dimethylallyl pyrophosphate; DPI, diphenylene iodonium; EGTA, ethylene glycol-bis-β
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Signal transduction and metabolic flux of β-thujaplicin and monoterpene biosynthesis in elicited Cupressus lusitanica cell cultures
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Publisher
Database: Elsevier - ScienceDirect
Journal: Metabolic Engineering - Volume 8, Issue 1, January 2006, Pages 14–29
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us