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Application of sequential integration for metabolic engineering of 1,2-propanediol production in yeast

Paper ID Volume ID Publish Year Pages File Format Full-Text
32009 44875 2006 8 PDF Available
Title
Application of sequential integration for metabolic engineering of 1,2-propanediol production in yeast
Abstract

The yeast species Saccharomyces cerevisiae was engineered to produce 1,2-propanediol (1,2-PD) using the δ/UB sequential gene integration method. To study the effects of increased copy number, 2 genes (mgs and gldA from Escherichia coli) were sequentially integrated into the chromosomes of S. cerevisiae strains of opposite mating type. The resulting strains (containing 0–3 copies of either mgs or gldA) were mated to create all possible combinations of the 2 genes introduced for 1,2-PD production. Enzyme activities were generally correlated with copy number, although there was greater variation in GldA activity in the diploid cells. The integrated genes were confirmed by Southern blot and 1,2-PD production was analyzed by HPLC. The strain containing 3 copies of mgs and gldA showed the highest level of 1,2-PD; however, 1,2-PD concentration was not clearly related to gene copy number. 1,2-PD production did correlate with Mgs specific activity, and high GldA specific activity was found to be inhibitory.

Keywords
Metabolic engineering; Saccharomyces cerevisiae; Gene integration; 1,2-Propanediol
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Application of sequential integration for metabolic engineering of 1,2-propanediol production in yeast
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Publisher
Database: Elsevier - ScienceDirect
Journal: Metabolic Engineering - Volume 8, Issue 1, January 2006, Pages 58–65
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
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